The artist has given the complete sum of his £25k Mercury Prize cash to the trust for venues, which functions to preserve the UK's independent music venues.
He was awarded the cheque on October 16 as his new release People Watching was chosen as the outstanding release of the previous year.
Fender has chosen to give the cash to the MVT, in acknowledgment of the essential function local spots played in his early career.
"I personally couldn't be pursuing this success if it weren't for all the gigs I performed around the region, and elsewhere, when I was beginning," stated Fender. "These venues are iconic, but they are facing challenges."
Beginning in 2023, more than 150 of these spaces have shut down – approximately 16 percent of the total UK scene.
Over the past year, top acts including Pulp, Coldplay, the singer Katy Perry and Ed Sheeran have all backed the Music Venues Trust in its efforts to help venues survive, by adding a minor surcharge to their admission fees, which benefits local stages.
Sam Fender also participated, generating over £100k on his live tour to support thirty-eight local spots across the UK.
The donation assisted spaces that were confronting imminent closure due to difficulties stemming from water damage, fire incidents and personal losses, as well as licensing issues, conflicts and noise complaints.
Other venues were granted financial assistance in modernizing infrastructure and sound and lighting systems that positively impacted musicians and concertgoers.
"The idea that money from performances in big venues backs the smaller venues, where it begins for performers like me, is just common sense," Sam Fender has stated.
The musician is among several artists to contribute his honor funds to worthy causes.
When the band won the award in 1996 for their release Different Class, frontman Jarvis Cocker stated that the band would give their prize money to the charity War Child.
In 2002, rapper and singer the artist shared her award among a few good causes, such as the NSPCC and a Sickle Cell charity.
"And I gave a grand to my local community centre, to their gymnastics class, because I did gymnastics when I was a child and they required updated gear," she shared with the media.
In recent years, they gave their funds to the youth organization that supported their act, as well as other independent music bodies.
Also 1994 winners the group donated their award to a multiple sclerosis charity after a friend was identified with the condition.
"Receiving the award was quite enough," stated artist the singer. "The cash was the bonus but we didn't require the extra, because we had the main prize. So our winning impacted somebody else's life."
Recently, the MVT announced it had rescued two grassroots venues in the South East, by placing them under community ownership.
The first in Southampton and The Croft in the city were bought under the public acquisition initiative, which is backed by Arts Council England and supporters who buy "shares" in the properties.